Gram stain lab report sample. Example of a Microbiology Unknown Lab Report 2019-02-11

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Gram staining lab report Research Paper Example : maximaconteudo.com.br

gram stain lab report sample

The purpose of this study was to identify a Gram positive and a Gram-negative bacterium from a mixed culture. This led to the Nitrate test with a positive result that narrowed it down to four bacteria. Indolent producers are bacteria that produce the enzyme transposes which can hydrology thyrotrophic to private, ammonia and indolent. A Gram stain was performed which identified Gram positive cocci. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Some stains can also be used to identify and classify bacteria. Upon reviewing the identification tables, the deciding biochemical test was the Casein test which tests for the production of the enzyme casease to break down the milk protein casein.

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Gram Negative Unknown Lab Report

gram stain lab report sample

In my case I had a gram negative bacteria. In all there were four different kinds of gram positive bacteria, two notably different types of gram negative bacteria, and only one gram insensitive bacteria. Gram negative bacteria cell walls have a thin peptidoglycan layer, in which an ample amount of lipids cross throughout the peptidoglycan and cell membrane. Gram staining is a very important technique used in biology labs all over the world. Saint Louis Community College at Meramec, 2011. This deduction was reached with several bits of evidence. Principle of Gram Stain Image 2: Cell wall of Gram Positive and Gram Negative Bacteria The differences in cell wall composition of Gram positive and Gram negative bacteria accounts for the Gram staining differences.

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Laboratory‎ > The Gram Stain and Simple Stain Smear

gram stain lab report sample

The next test performed was a Casein Test which showed a clear positive result. It was determined that it was a Gram negative rod. Wash with tap water at the end of the 30 seconds to stop the decolorization. Kenyon College, 30 June 2006. It is suggested that culture 11 is a sample of Enterobacter aerogenes. A Gram stain was performed and identified as Gram negative rods.

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Gram Negative Unknown Lab Report

gram stain lab report sample

Keep coughing up sputum until the cup is filled to the marker, which should equal approximately 1 teaspoon. One drop of water was dropped to the clean slide by using sterile loop. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. Iodine I — or I3 — acts as a mordant and as a trapping agent. A Nitrate test was performed to confirm the result of E. Wash, drain, blot, and examine under oil.

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Gram staining lab report free essay sample

gram stain lab report sample

Lab Manual for General Microbiology. Gloves are worn for the same purpose. Light microscope employs visible light to detect small objects. Coli is a bacterium that is prevalent in the gut of warm blooded organisms. The urea hydrolysis test determines which organisms are able or unable to hydrolyze urea with the enzyme urease. This step is critical and must be timed correctly otherwise the crystal violet stain will be removed from the Gram-positive cells. This was a positive result for mannitol fermentation which narrowed it down to two bacteria.

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Microbiology Laboratory Report Essay Example for Free

gram stain lab report sample

Discussion There were many good specimens that ended up beautifully stained. It has special techniques and optics thus it can reveal the structure and biochemistry of living cells. {Colonies are large, beige or cream-colored, with irregular borders. The urea hydrolysis test can yield four possible results: rapid urease-strong urease production-positive, slow urease-weak urease production- weak positive, and urease absent-no urease hydrolysis-negative bacteria. Results The gram staining lab was a huge success. This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope.

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Example of Unknown Lab Report, Microbiology

gram stain lab report sample

The tiny nature of microbes has revolutionized the use of stains to aid in having a vivid and transparent picture of microbes under microscope Ryu 60. This result was confirmed by Lab instructor. The sterile techniques and methods were followed using the laboratory manual by McDonald et. Expected experimental result, Escherichia coli E. This is a statement, which can be justified by numerous researches carried out without success. This was the correct identification because all of the other Gram negative were eliminated. Gram positive purple cocci bacteria were identified using a microscope.

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Gram stain lab report

gram stain lab report sample

This positive test means that the unknown bacteria is capable of performing mixed acid fermentation. Between these two pH values, it is various shades of orange Leboffe and Pierce 161. First, a sulfide-indole-motility agar deep was inoculated with bacteria from the MacConkey plate. The results were Gram positive cocci. Light is conducted through curve lenses in such a way that an object may be viewed larger than its actual size.

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